Fluorescence Microscopy


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eNewsletter - November 2007

precisExcite® LED excitation - Simply Better Control
precisExcite® excitation offers Control, Stability, and Homogeneity for fluorescence microscopy using high-power LED Arrays.  The unit is modular, allowing selection of wavelengths, and can be fitted to all current microscopes.



precisExcite® - Latest News:-
LAMs @ 445 & 505nm   -   Dual Staining   -   ASCB, Washington


      LED Array Modules : 445nm & 505nm

CoolLED is pleased to announce two new LED Array Modules (LAMs) for the precisExcite LED source.  These are at 445nm (CFP) and 505nm (YFP) wavelengths.  These complement the existing wavelengths available at 400, 465, 525, 595 and 635nm.  Full details and optimal filter data can be found at the precisExcite website.  

precisExcite uses high-power LED arrays which benefit from active cooling to achieve precise thermal control.  This ensures maximum intensity and stability throughout their lifetime.   CoolLED is constantly monitoring LED availability and will continue to introduce new wavelengths as they become available.

Click for more information on LAMs and Filters

 


 


     Dual Staining - GFP & mCherry

Dual staining applications such as GFP + mCherry are becoming common procedure. The ability to control the intensity of individual wavelengths independently means that excitation can be balanced such that one strong fluorophore is not saturating a camera, whilst another is seen as too weak.  ND filters and shutters are not required when using precisExcite for this procedure.

The precisExcite unit can be specifically configured for this application.  Excitation filters can be integrated within the system and an optimised double dichroic supplied.  This mean that there will be no moving parts, resulting in vibration-free microsecond switching.

Please contact CoolLED if you are interested in further details on this application.

Click for more information on Dual Staining
    


  American Society for Cell Biology meeting, 
  Washington DC, 2-4 December

        CoolLED will be exhibiting at the ASCB meeting at the beginning 
        of December.  This will be an opportunity to see precisExcite 
        units and appreciate the level of control and stability that is 
        now possible for your fluorescence excitation.  CoolLED will be
        at Booth 1045.

            For more information on ASCB meeting


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