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Stock Filters You can identify the best configuration of precisExcite from information in Applications or Configurations. The tables below will help you to identify the best stock filters available from current suppliers for the configuration you have selected. Alternatively, you can specify the filters from the Ideal Filters page. Check the precisExcite LED options chart to see if LAMs are available in the excitations regions in which you are currently working.
The tables below include direct links to the respective manufacturer's website where you can see spectral data. The lists do not identify all suppliers or possible filter combinations and is intended purely as an aid. Please contact CoolLED for assistance in selecting and sourcing filters for your precisExcite system. 1. Single Band Filter Sets For use in all precisExcite system configuration.
2. Multiband Filter Sets For use in the pE-2 configuration for Dual Staining / Live Cell applications This table identifies a complete filter set comprising a dual-wavelength excitation, dual-wavelength dichroic and dual-wavelength emission filter which will be fitted in a single filter cube within the microscope.
3. Alternatives to Filter-wheel arrangements For use in the pE-2 configuration for fast-switching & vibration-free applications Conventional lamp-based excitation systems use “Pinkel” or “Sedat” filter sets for fast-switching applications. In the case of Pinkel sets, these consist of individual excitation filters which are held in an excitation filter wheel arrangement with a multiband dichroic and emission filter in the microscope cube. In the case of Sedat sets, the multiband emission filter is also replaced by single band filters in a second filter wheel. The flexibility of the pE-2 configuration is that the collimator will accept excitation filters within the collimator body. When used in conjunction with a Pinkel filter set, a fast-switching (microsecond using TTL control) arrangement with no moving parts is possible. This will have no mechanical delay or vibration.
NOTE: For optimum performance, the pE-2 collimator uses 35mm diameter excitation filters. Users may consider using standard 25mm diameter excitation filters. In this instance, it is possible that intensity may be reduced and some vignetting within the field-of-view may be observed. This is unlikely to effect performance when using a camera but may be observed through the eyepiece. |
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