Fluorescence Microscopy


Stock Filters

You can identify the best configuration of precisExcite from information in Applications or Configurations. The tables below will help you to identify the best stock filters available from current suppliers for the configuration you have selected. Alternatively, you can specify the filters from the Ideal Filters page.

Check the precisExcite LED options chart to see if LAMs are available in the excitations regions in which you are currently working.

The tables below include direct links to the respective manufacturer's website where you can see spectral data. The lists do not identify all suppliers or possible filter combinations and is intended purely as an aid. Please contact CoolLED for assistance in selecting and sourcing filters for your precisExcite system.


1. Single Band Filter Sets

For use in all precisExcite system configuration.

This table identifies a complete filter set comprising an excitation, dichroic and emission (EX/DI/EM) filter. These will match the selected LED excitation wavelengths. In most cases there are several options. You can select by looking at the performance data on the respective manufacturer's website.

SINGLE BAND FILTER SETS
LAM Wavelength
Typical Fluorophores
Chroma Part #
Semrock part#
 
 
 
 
400nm
DAPI
 
445nm
CFP
465nm
FITC, GFP

GFP-A-BASIC-000
FITC-A-Basic-000
FITC-LP01-Clinical-000
GFP-3035B-000
FITC-3540B-000

490nm
GFP
 
505nm
YFP
525nm
TRITC
535nm
TRITC, Cy3
565nm
TxRed, Cy3.5, mCherry
585nm
mCherry, TxRed, Alexa 594
 
595nm
mCherry, Alexa 594
 
635nm
Cy5
TBA
 
 
 


2. Multiband Filter Sets

For use in the pE-2 configuration for Dual Staining / Live Cell applications This table identifies a complete filter set comprising a dual-wavelength excitation, dual-wavelength dichroic and dual-wavelength emission filter which will be fitted in a single filter cube within the microscope.

MULTIBAND FILTER SETS
LAM
Wavelength
Typical fluorophores Filter set
Part number

465nm OR 490nm
+
585nm

GFP/mCherry
FITC/Texas Red

Semrock   - FITC/TXRED-A-000

Chroma - 59022

445nm
+
505nm

CFP/YFP

Semrock Part - CFP/YFP-A-000

Chroma Part - 59017

465nm
+
535nm OR 565nm

FITC/TRITC

Semrock Part – GFP/DsRED-A-000

525nm  OR 535nm
+
635nm

Cy3/Cy5 Semrock Part – CY3/CY5-A-000

400/490nm
+
565nm

DAPI/FITC/Texas Red

Semrock Part – DA/FI/TB-B-000

Chroma Part - 69002


3. Alternatives to Filter-wheel arrangements

For use in the pE-2 configuration for fast-switching & vibration-free applications

Conventional lamp-based excitation systems use “Pinkel” or “Sedat” filter sets for fast-switching applications.  In the case of Pinkel sets, these consist of individual excitation filters which are held in an excitation filter wheel arrangement with a multiband dichroic and emission filter in the microscope cube.  In the case of Sedat sets, the multiband emission filter is also replaced by single band filters in a second filter wheel.

The flexibility of the pE-2 configuration is that the collimator will accept excitation filters within the collimator body.  When used in conjunction with a Pinkel filter set, a fast-switching (microsecond using TTL control) arrangement with no moving parts is possible.  This will have no mechanical delay or vibration.

FAST-SWITCHING, VIBRATION-FREE SETS
LAM
Wavelength
Typical fluorophores Suitable 'Pinkel' set
Part numbers

465nm
+
585nm

GFP/mCherry
FITC/Texas Red

Semrock Part – GFP/HcRed-2X-A-000
(Request alternative excitation filter for
GFP : FF01-465/30)

490nm
+
585nm

GFP/mCherry
FITC/Texas Red

Semrock Part – GFP/HcRed-2X-A-000

 Chroma Part – 59222

445nm
+
505nm

CFP/YFP

Chroma Part - 59217

465nm
+
565nm

FITC/TRITC Semrock Part – GFP/DsRed-2-A-000

NOTE: For optimum performance, the pE-2 collimator uses 35mm diameter excitation filters. Users may consider using standard 25mm diameter excitation filters. In this instance, it is possible that intensity may be reduced and some vignetting within the field-of-view may be observed. This is unlikely to effect performance when using a camera but may be observed through the eyepiece.

 

fluorescence microscopy
 

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