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precisExcite® & pE-2 Collimator
The precisExcite pE-2 collimator has been developed to offer greater flexibility with the incorporation of interchangeable high-performance optics within the collimator itself. The selection of the pE-2 collimator with the precisExcite main unit will be particularly beneficial for Live Cell applications. Fast-switching (in microseconds) of excitation wavelengths without any moving parts can be achieved by selecting appropriate multi-band excitation, dichroic and emission filters for the microscope’s filter cube. In addition, it is possible to fit excitation filters within the pE-2 collimator for applications requiring the highest performance.
Dual staining and other Live Cell applications can be conducted with reduced phototoxicity due to the cleaner light, incredible stability and shortened switching times from an LED source, resulting in more frequent imaging and better data.
pE-2 Combiner
As shown, the pE-2 Collimator is configured for maximum flexibility with the ability to exchange wavelength combining dichroics to give optimum performance with the wide range of LAMs and multi-bandpass Filter sets available. These Combiners provide spectral filtering appropriate for eliminating cross excitation when used with multiband applications. The exchangeable Combiner allows selection from the full range of LAMs.
Consolidating the filters within precisExcite and the microscope cube
The two graphics below show how the filtering and mechanical arrangements of a conventional illumination system can be replaced using the pE-2 collimator arrangements of the precisExcite LED source.
Filter Selection
The multi-bandpass filter sets which can be used in conjunction with the pE-2 collimator fit in the microscope manufacturers' single filter cube to allow high speed microsecond switching with no moving parts.
When multiple fluorophore emissions are intended to be seen simultaneously, multi-bandpass excitation filters as well as multi-bandpass dichroics and emission filters are typically used. This is because of the broad spectrum from traditional lamp sources.
However, LEDs have relatively narrow bandwidths with only very weak "tails" that require filtering. The exchangeable Combiner in the pE-2 Collimator performs this filtering function, allowing alternate excitation wavelength switching for high resolution sequential imaging with single black & white camera systems.
For information on multi-bandpass Filter sets.
Filter wheels
Conventional lamp-based excitation systems use "Pinkel" or "Sedat" filter sets for fast-switching applications. In the case of Pinkel sets, these consist of individual excitation filters which are held in an excitation filter wheel arrangement with a multiband dichroic and emission filter in the microscope cube. In the case of Sedat sets, the multiband emission filter is also replaced by single band filters in a second filter wheel.
The flexibility of the pE-2 configuration is that the collimator will accept excitation filters within the collimator body. When used in conjunction with a Pinkel filter set, a fast-switching (microsecond using TTL control) arrangement with no moving parts is possible. This will have no heat, mechanical delay or vibration.
For information on "Pinkel" Filter sets.
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