Time Lapse Microscopy describes all imaging of the same sample (e.g., a cell) which is photographed at regular time intervals over a defined period.

In order to achieve meaningful results, the imaging parameters need to be reliable and consistent.  Using conventional Mercury/Halide lamps, there are problems with lamp intensity varying over time.  Also, heat from the lamps can cause focus drift which compromises results.  Lamps have a limited lifetime and may need to be replaced during the experiment.  Alignment difficulties and the different intensity of a new lamp will effect results.

These problems are overcome using precisExcite. 

• precisExcite switches on/off instantly
• precisExcite provides same intensity during experiment
• precisExcite only excites in the waveband required

In particular, Live Cell imaging of samples will be affected by the amounts of phototoxicity and photobleaching from the light source and of the fluorophore.  precisExcite can reduce these problems due to the narrowband of excitation and the dramatically reduced levels of UV intensity of LEDs.

Typical Live Cell configurations using precisExcite® are achieved with the pE-2 collimator.