Dual Staining is a typical Live Cell imaging application.  It is an important and growing area of fluorescence microscopy.  In order to understand and define the role of the vast number of proteins in the cellular processes associated with health and disease, it is preferable to examine proteins in living cells themselves as this offers insights not possible using fixed cell techniques.

Today's live cell imagers can offer the resolution required to image living cells without destroying them.  In addition, developments in fluorescent dyes and proteins have further facilitated the study of complex cellular processes using fluorescent staining ("labelling") of various proteins, ions and lipids in living cells.  However, the types and duration of cellular processes that can be imaged have been constrained in microscopy systems by limitations in acquisition speed (camera), mechanical delay (shutters, filters), and by phototoxicity and photobleaching (light sources). 

Cameras are now available which offer high acquisition speeds.  The remaining problems are mitigated using precisExcite.

• A Live Cell imaging application


• Better conducted without vibration caused by moving parts


• Optimised where intensity levels are set independently for each fluorophore


• precisExcite offers these benefits